Title

ADAR1 restricts LINE-1 retrotransposition

Main Authors

Elisa Orecchini; Alessandro Michienzi

Main institution

University of Rome ‘Tor Vergata’

Read

September 30, 2016

Link to the manuscript

Notes

Intro:

RNA editing proteins (ADARs) can restrict (antiviral) or be exploited (proviral) by (endogenous and exogenous) virus.

Results:

ADAR1 is associated with many protein related to the regulation of L1 activity. Namely Orecchini et al tested the interactions between ADAR1 and:

Next Orecchini et al tested if the knock out of ADAR1 influences the retrotransposition of L1s and found that "knockdown of ADAR1 expression in HeLa cells increased L1 activity by 2- to 3-fold when compared to controls (scramble shRNA and empty vector)". Other vectors were tested and similar results were found. "Knockdown of ADAR1 expression in HeLa cells increased L1 retrotransposition efficiency by at least 6-fold when compared to the controls."

Over expression of ADAR1 results is less ensuring. From Figure5, we can learn that the empty vector is over expressed, the Fluc/Rluc ration is 0.025, or 2.5%. Which in turn could be roughly interpreted as “the proportion of transfected vectors mobilized". When ADAR1 is over-expressed, the Fluc/Rluc is reduced to 1% or .5%. Indeed, there is a reduction of about 50%, however, both are extremely small proportions.

From Co-immunopreciptation Orecchini learn that: